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Age 11-14 (KS3)
Age 14-16 (KS4 / GCSE)

A practical adapted from A-level work, used by year 7 students. Uses chromatography to explore photosynthesis in red plants. Step by step instructions on the PowerPoint with separate teacher's and technician's notes.

Published 28 Jun 2009

Contributed by Jan Stevens — Experienced contributor

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Abigail Laing - Admin

It's always good to have materials ready for practical lessons - this lists equipment and has step-by-step instructions.

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Related Resources

Other resources listed under Biology / Plants / Age 11-14 (KS3) / Age 14-16 (KS4 / GCSE) /

Extracts from this resource

Can red plants photosynthesise

1Teachable.net 2009. Some rights reserved.
http://teachable.net/res.asp?r=2736 Can red plants
photosynthesise?2ChromatographyYou all know that green leaves contain chlorophyll. Chlorophyll is known as a plant pigment.
Chlorophyll helps a plant to photosynthesise.
How can plants with red leaves photosynthesise? Do they have chlorophyll?
Is chlorophyll a simple substance or is it made of more than one pigment?3ChromatographyYou will have seen your teacher demonstrate what happens with ink. The ink is made up of more than one pigment.
You are going to repeat this experiment using red and green leaves.4Equipment You will work in pairs. One of you will use a red leaf and one a green leaf.
Each person will need
A boiling tube with a cork and pin attached
A leaf
A pencil and a ruler
Some solvent
Safety glasses
Access to a mortar and pestle- it may be necessary to share these in groups.
A boiling tube rack one between two.
A small bottle of acetone5MethodWatch carefully as your teacher shows you what to

Teachers notes for Chromatography lesson

Teachers notes for Chromatography lesson
The purpose of this lesson is to use chromatography to demonstrate that chlorophyll is made of more than one substance and thus red leaves can photosynthesise because they have these pigments.
You can point out to students that the reason leaves change colour in the autumn is that each pigment is broken down in turn leaving the others to show up.
Students could use felt pens and water to do a simple chromatography practical before using leaves. This may be more important for groups who have not done chemistry lately.
Demonstrate the technique using a black felt tip pen. Draw a pencil line 30mm from the bottom of a strip of filter paper (discuss why this is in pencil not pen).
Prepare a spot of ink on the line- demonstrating the need to build the spot slowly
Place small amount of water in the bottom of a boiling tube and place strip

Creative Commons License

This work is licensed under a Creative Commons License